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Addgene

pXIP TGBRI K to R
(Plasmid #19150)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19150 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQCXIP
  • Backbone size w/o insert (bp) 7200
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Grow in STBL3 cells
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Transforming growth factor, beta receptor I
  • Alt name
    TGFBR1, AAT5, ACVRLK4, ALK-5, ALK5, LDS1A, LDS2A, SKR4, TGFR-1
  • Species
    H. sapiens (human)
  • Mutation
    K420R mutation
  • Entrez Gene
    TGFBR2 (a.k.a. AAT3, FAA3, LDS1B, LDS2, LDS2B, MFS2, RIIC, TAAD2, TBR-ii, TBRII, TGFR-2, TGFbeta-RII, tbetaR-II)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV Forward
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Wei He

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pXIP TGBRI K to R was a gift from Joan Massague (Addgene plasmid # 19150 ; http://n2t.net/addgene:19150 ; RRID:Addgene_19150)

  • For your References section:

    TGFbeta primes breast tumors for lung metastasis seeding through angiopoietin-like 4. Padua D, Zhang XH, Wang Q, Nadal C, Gerald WL, Gomis RR, Massague J. Cell. 2008 Apr 4. 133(1):66-77. 10.1016/j.cell.2008.01.046 PubMed 18394990
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