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Addgene

pAsp-AMA-gpdA-ptr
(Plasmid #191016)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191016 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPTRII
  • Backbone manufacturer
    TaKaRa
  • Backbone size (bp) 10031
  • Modifications to backbone
    Removal of non-functional sequence of AMA1 in pPTRII, expression of cas9 using Aspergillus nidulans gpdA promoter and trpC terminator, inclusion of unique PstI and KpnI sites for cloning Aspergillus flavus U6 promotor and terminator based sgDNAs.
  • Vector type
    Unspecified ; Aspergillus

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAsp-AMA-gpdA-ptr was a gift from Perng-Kuang Chang (Addgene plasmid # 191016 ; http://n2t.net/addgene:191016 ; RRID:Addgene_191016)

  • For your References section:

    A Simple CRISPR/Cas9 System for Efficiently Targeting Genes of Aspergillus Section Flavi Species, Aspergillus nidulans, Aspergillus fumigatus, Aspergillus terreus, and Aspergillus niger. Chang PK. Microbiol Spectr. 2023 Feb 14;11(1):e0464822. doi: 10.1128/spectrum.04648-22. Epub 2023 Jan 18. 10.1128/spectrum.04648-22 PubMed 36651760
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