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Addgene

Anti-GABA(A)R, Alpha2 [N399/19R]
(Antibody #190894)

Ordering

Item Catalog # Description Quantity Price (USD)
Recombinant Antibody 190894-rAb 100 µg of purified recombinant antibody $250 *
Recombinant Antibody trial size 190894-rAb.T 20 µg of purified recombinant antibody $85 *

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Features

Production & Usage

  • Production Plasmid
    Anti-GABA(A)R, Alpha2 [N399/19R]
  • Purification
    Purified from cell culture supernatant using Protein A or Protein G affinity columns followed by a buffer exchange with centrifugal columns.
  • Storage Buffer
    PBS + 1 mM sodium azide

Delivery

  • Concentration
    0.9–1.1 mg/mL
  • Storage
    Upon receipt, prepare single use aliquots and store at -20 °C until use. Avoid multiple freeze thaw cycles.
  • Shipment
    Blue ice

Terms and Licenses

Target Antigen

Protein Gamma-aminobutyric acid receptor subunit alpha-2
Antigen Description Amino acids 350–385 (cytoplasmic C-terminus) of rat GABA-A-R-Alpha2
Antigen Amino Acid Sequence
VNDKKKEKGSVMIQNNAYAVAVANYAPNLSKDPVLS
Species R. norvegicus (rat)
Additional Species Reactivity M. musculus (mouse)
Gene Gabra2
Alternative Names
  • GABA(A) receptor subunit alpha-2
External References

Applications (1)

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Western Blot

1 image
Western Blot image by Uwe Rudolph, Harvard Medical School Knockout validation
Submitted By: Uwe Rudolph, Harvard Medical School
Antibody Type
Hybridoma
Sample Species
R. norvegicus (rat)
Result
Pass

Depositor Comments

Does not cross-react with GABA-A-R-Alpha1
How to cite this antibody ( Back to top)

These antibodies were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the antibodies were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Anti-GABA(A)R, Alpha2 [N399/19R] - from James Trimmer (Addgene antibody # 190894 ; http://n2t.net/addgene:190894)
  • For your References section:

    A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. Andrews NP, Boeckman JX, Manning CF, Nguyen JT, Bechtold H, Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS. Elife. 2019 Jan 22;8. pii: 43322. doi: 10.7554/eLife.43322. 10.7554/eLife.43322 PubMed 30667360