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α-actinin-4-M522-Venus in pcDNA6
(Plasmid #190802)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190802 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA6 myc-HisA
  • Backbone size w/o insert (bp) 5126
  • Total vector size (bp) 8583
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    alpha-actiinin-4-M522-venus
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    3600
  • GenBank ID
    XM_017027331.3 ACTN4
  • Entrez Gene
    ACTN4 (a.k.a. ACTININ-4, FSGS, FSGS1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • venus
    • myc (C terminal on insert)
    • His (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer Clone EZ, Genscript
  • 3′ sequencing primer Clone EZ, Genscript
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    α-actinin-4-M522-Venus in pcDNA6 was a gift from Vivian Tang (Addgene plasmid # 190802 ; http://n2t.net/addgene:190802 ; RRID:Addgene_190802)

  • For your References section:

    Synaptopodin stress fiber and contractomere at the epithelial junction. Morris T, Sue E, Geniesse C, Brieher WM, Tang VW. J Cell Biol. 2022 May 2;221(5). pii: 213132. doi: 10.1083/jcb.202011162. Epub 2022 Apr 13. 10.1083/jcb.202011162 PubMed 35416930
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