CYBL-SPI1-CEBPB-IRF5
(Plasmid #190719)

• Purpose: For microglia differentiation from iPSC
• Depositing Lab: Li Gan
• Publication: Drager et al Nat Neurosci. 2022 Aug 11. pii: 10.1038/s41593-022-01131-4. doi: 10.1038/s41593-022-01131-4.

Backbone

• Vector backbone: PUCM
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SPI1-CEBPB-IRF5
• Species: H. sapiens (human)
• Insert Size (bp): 3499

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • CYBL-SPI1-CEBP-IRF5 (Good)with annotations.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note our NGS result found a few ambiguous bases in a repetitive region of the insert between the T2A sites. These ambiguous bases find a mixed population at these base pairs, some will affect the amino acid sequence.