pABCb10aa1-35-GFP
(Plasmid #19035)

• Depositing Lab: Orian Shirihai
• Publication: Graf et al J Biol Chem. 2004 Oct 8. 279(41):42954-63.

Backbone

• Vector backbone: pEGFP N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: amino acids 1-35 of the mitochondrial targeting sequence of ABCb10
• Species: M. musculus (mouse)
• Insert Size (bp): 35
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: BamH1 (not destroyed)
• 5′ sequencing primer: EGFP-N Sequencing Primer (#6479-1): 745–724
• 3′ sequencing primer: EGFP-C Sequencing Primer (#6478-1):

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry