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Addgene

pAV105-PPAB1-GFP-Erk1R84S-1SIM
(Plasmid #190253)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190253 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Pav105
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ERK1R84S-SIM
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    2127
  • Mutation
    ERK1R84S
  • Tag / Fusion Protein
    • GFP

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAV105-PPAB1-GFP-Erk1R84S-1SIM was a gift from Liam Holt (Addgene plasmid # 190253 ; http://n2t.net/addgene:190253 ; RRID:Addgene_190253)

  • For your References section:

    Condensed-phase signaling can expand kinase specificity and respond to macromolecular crowding. Sang D, Shu T, Pantoja CF, Ibanez de Opakua A, Zweckstetter M, Holt LJ. Mol Cell. 2022 Oct 6;82(19):3693-3711.e10. doi: 10.1016/j.molcel.2022.08.016. Epub 2022 Sep 14. 10.1016/j.molcel.2022.08.016 PubMed 36108633
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