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Addgene

pMB1-A-pBAD-dCas9
(Plasmid #190132)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190132 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMB1-A
  • Backbone manufacturer
    Paul Freemont Lab / iGem
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9
  • Species
    S. pyogenes

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Removed BsmBI site

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMB1-A-pBAD-dCas9 was a gift from Veronica Godoy-Carter (Addgene plasmid # 190132 ; http://n2t.net/addgene:190132 ; RRID:Addgene_190132)

  • For your References section:

    AspFlex: Molecular Tools to Study Gene Expression and Regulation in Acinetobacter baumannii. Brychcy M, Kokodynski A, Lloyd D, Godoy VG. ACS Synth Biol. 2023 Aug 16. doi: 10.1021/acssynbio.3c00167. 10.1021/acssynbio.3c00167 PubMed 37587063
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