pFBP_2xNbLYS1::LUZ
(Plasmid #189918)

• Purpose: Binary vector for Agrobacterium-mediated transient expression of a bioluminescent reporter for assessing immune activation in Nicotiana benthamiana by transiently expressed plant immune receptors.
• Depositing Lab: Adam Steinbrenner
• Publication: Garcia et al Mol Plant Microbe Interact. 2023 Mar;36(3):139-149. doi: 10.1094/MPMI-07-22-0160-TA. Epub 2023 Feb 7.

Backbone

• Vector backbone: pTRANS_220d
• Total vector size (bp): 25263
• Vector type: Plant Expression, Luciferase
• Selectable markers: Kanamycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p2xNbLYS1:NnLuz:tAtuOcs
• Species: Neonothopanus nambi, Nicotiana benthamiana, Aspergillus nidulans
• Promoter: Various

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsaI (destroyed during cloning)
• 3′ cloning site: BsaI (destroyed during cloning)
• 5′ sequencing primer: ttacactagctgcgttgg
• 3′ sequencing primer: gctcttgtcggaacaagc

Resource Information

• Supplemental Documents:
  • pFBP_2xNbLYS1_LUZ.gb
• A portion of this plasmid was derived from a plasmid made by: Building customizable auto-luminescent luciferase-based reporters in plants. Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson R, Rizvi F, Imaizumi T, Voytas DF. Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786. 10.7554/eLife.52786 PubMed 32209230 -- Received as a gift from Daniel Voytas (Addgene plasmid # 139697)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2022.07.28.501876v1 for bioRxiv preprint.