Cherry-LacRep
(Plasmid #18985)

• Depositing Lab: Mirek Dundr
• Publication: Dundr et al J Cell Biol. 2007 Dec 17. 179(6):1095-103.

Backbone

• Vector backbone: pEGFP-C1
• Backbone size w/o insert (bp): 4700
• Modifications to backbone: EGFP was switched with mCherry using NheI and BsRGI sites.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LacI
• Alt name: Lac Repressor
• Promoter: CMV
• Tags / Fusion Proteins:
  • mCherry (N terminal on backbone)
  • SV40 NLS (C terminal on insert)
  • AviTag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Articles Citing this Plasmid:
  • 13 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Also described in Science 13 June 2008 320: 1507-1510

"It was cloned into pEGFP-C1 vector (Clontech) where GFP was switched with mCherry using NheI and BsRGI sites. Lac Repressor-NLS was inserted into this vector using XhoI and KpnI sites."