pRnc-gGFP161
(Plasmid
#189536)
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PurposeExpresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-161-GFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 189536 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBR322
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 4660
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameRNase III
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Alt namernc
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SpeciesE. coli
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Insert Size (bp)678
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MutationN/A
- Promoter pBAD
Cloning Information for Gene/Insert 1
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (not destroyed)
- 3′ cloning site SacI (not destroyed)
- 5′ sequencing primer pBad-Forward
- 3′ sequencing primer pBad-Reverse (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert namegUTR-161-GFP
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Alt namefusion of rnc UTR and gRNA targeting GFP
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Insert Size (bp)256
- Promoter J23119
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byAddgene - pgRNA (bacterial): Plasmid #44251
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRnc-gGFP161 was a gift from Marc Ostermeier (Addgene plasmid # 189536 ; http://n2t.net/addgene:189536 ; RRID:Addgene_189536) -
For your References section:
A CRISPR-dCas9 System for Assaying and Selecting for RNase III Activity In Vivo in Escherichia coli. Hauk P, Weeks R, Ostermeier M. CRISPR J. 2022 Dec 9. doi: 10.1089/crispr.2022.0041. 10.1089/crispr.2022.0041 PubMed 36493370