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Addgene

pRnc-gGFP161
(Plasmid #189536)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 189536 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBR322
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 4660
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    RNase III
  • Alt name
    rnc
  • Species
    E. coli
  • Insert Size (bp)
    678
  • Mutation
    N/A
  • Promoter pBAD

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer pBad-Forward
  • 3′ sequencing primer pBad-Reverse
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    gUTR-161-GFP
  • Alt name
    fusion of rnc UTR and gRNA targeting GFP
  • Insert Size (bp)
    256
  • Promoter J23119

Cloning Information for Gene/Insert 2

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Addgene - pgRNA (bacterial): Plasmid #44251

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRnc-gGFP161 was a gift from Marc Ostermeier (Addgene plasmid # 189536 ; http://n2t.net/addgene:189536 ; RRID:Addgene_189536)
  • For your References section:

    A CRISPR-dCas9 System for Assaying and Selecting for RNase III Activity In Vivo in Escherichia coli. Hauk P, Weeks R, Ostermeier M. CRISPR J. 2022 Dec 9. doi: 10.1089/crispr.2022.0041. 10.1089/crispr.2022.0041 PubMed 36493370