pMSCV mKeima-LC3
(Plasmid
#189006)
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PurposeFluorescent reporter for autophagy
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 189006 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMSCV PGK-neo
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Vector typeRetroviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemKeima, LC3
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Alt nameMap1lc3b
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SpeciesR. norvegicus (rat)
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Insert Size (bp)1113
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GenBank IDXM_017601351.1
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Entrez GeneMap1lc3b (a.k.a. LC3B, Map1lc3, Mpl3, zbs559)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.02.22.481456v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMSCV mKeima-LC3 was a gift from Atsushi Hoshino (Addgene plasmid # 189006 ; http://n2t.net/addgene:189006 ; RRID:Addgene_189006) -
For your References section:
Liver lipophagy ameliorates nonalcoholic steatohepatitis through extracellular lipid secretion. Minami Y, Hoshino A, Higuchi Y, Hamaguchi M, Kaneko Y, Kirita Y, Taminishi S, Nishiji T, Taruno A, Fukui M, Arany Z, Matoba S. Nat Commun. 2023 Jul 13;14(1):4084. doi: 10.1038/s41467-023-39404-6. 10.1038/s41467-023-39404-6 PubMed 37443159