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L444P GBA pcDNA3.1
(Plasmid #188582)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188582 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA 3.1 (+)
  • Backbone manufacturer
    ThermoFisher
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GBA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1750
  • Mutation
    L444P
  • Entrez Gene
    GBA1 (a.k.a. GBA, GCB, GLUC)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (5', carrying its own ATG) (unknown if destroyed)
  • 3′ cloning site XbaI (3', carrying its own TGA) (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    L444P GBA pcDNA3.1 was a gift from Anthony Schapira (Addgene plasmid # 188582 ; http://n2t.net/addgene:188582 ; RRID:Addgene_188582)

  • For your References section:

    The GBA variant E326K is associated with alpha-synuclein aggregation and lipid droplet accumulation in human cell lines. Smith LJ, Bolsinger MM, Chau KY, Gegg ME, Schapira AHV. Hum Mol Genet. 2022 Sep 20:ddac233. doi: 10.1093/hmg/ddac233. 10.1093/hmg/ddac233 PubMed 36130205
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