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pSPCas9(BB)-2A-GFP_ABCA3GFP_targeting
(Plasmid #188541)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188541 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSPCas9(BB)-2A-GFP
  • Backbone size w/o insert (bp) 9279
  • Total vector size (bp) 9292
  • Modifications to backbone
    n/a
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    gRNA
  • gRNA/shRNA sequence
    TCATCGCCCCTCCTCTGCGGcGG
  • Species
    H. sapiens (human)
  • Promoter U6
  • Tag / Fusion Protein
    • GFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (destroyed during cloning)
  • 3′ cloning site BbsI (destroyed during cloning)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSPCas9(BB)-2A-GFP_ABCA3GFP_targeting was a gift from Darrell Kotton (Addgene plasmid # 188541 ; http://n2t.net/addgene:188541 ; RRID:Addgene_188541)

  • For your References section:

    Heterogeneity in Human Induced Pluripotent Stem Cell-derived Alveolar Epithelial Type II Cells Revealed with ABCA3/SFTPC Reporters. Sun YL, Hurley K, Villacorta-Martin C, Huang J, Hinds A, Gopalan K, Caballero IS, Russo SJ, Kitzmiller JA, Whitsett JA, Beers MF, Kotton DN. Am J Respir Cell Mol Biol. 2021 Oct;65(4):442-460. doi: 10.1165/rcmb.2020-0259OC. 10.1165/rcmb.2020-0259OC PubMed 34101541
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