pHG4
(Plasmid
#188252)
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Purpose(Empty Backbone) Integrating plasmid contains the TG1 int/attP locus and the apramycin-resistance gene (aac(3)IV) for selection
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 188252 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBF20
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Vector typeBacterial Expression
- Promoter act1p/actII-orf4
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHG4 was a gift from Hong Gao & Maggie Smith (Addgene plasmid # 188252 ; http://n2t.net/addgene:188252 ; RRID:Addgene_188252) -
For your References section:
Integrating vectors for genetic studies in the rare Actinomycete Amycolatopsis marina. Gao H, Murugesan B, Hossbach J, Evans SK, Stark WM, Smith MCM. BMC Biotechnol. 2019 Jun 4;19(1):32. doi: 10.1186/s12896-019-0521-y. 10.1186/s12896-019-0521-y PubMed 31164159
Map uploaded by the depositor.
