LaminA-ECFP-SNAP
(Plasmid
#187965)
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Purposeexpresses LaminA with C-terminal tags ECFP and SNAP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 187965 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepECFP-SNAP
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Backbone manufacturerLabor Prof. Carsten Schultz, EMBL Heidelberg
- Backbone size w/o insert (bp) 6601
- Total vector size (bp) 8588
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Pir1
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLaminA
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Alt nameLMNA
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Alt nameLMN1
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SpeciesH. sapiens (human)
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Insert Size (bp)1987
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GenBank IDNM_001406983.1 NM_001406983.1
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Entrez GeneLMNA (a.k.a. CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1)
- Promoter CMV
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Tag
/ Fusion Protein
- ECFP-SNAP (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CMV-for (Microsynth)
- 3′ sequencing primer EGFP-N-rev (Microsynth) (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byordered from Addgene No55068
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LaminA-ECFP-SNAP was a gift from Markus Wahl (Addgene plasmid # 187965 ; http://n2t.net/addgene:187965 ; RRID:Addgene_187965) -
For your References section:
Recruitment of a splicing factor to the nuclear lamina for its inactivation. Vester K, Preussner M, Holton N, Feng S, Schultz C, Heyd F, Wahl MC. Commun Biol. 2022 Jul 22;5(1):736. doi: 10.1038/s42003-022-03689-y. 10.1038/s42003-022-03689-y PubMed 35869234