Halo-OPTN
(Plasmid #187900)

• Purpose: Mammalian cell expression of Halo-tagged OPTN
• Depositing Lab: Erika Holzbaur
• Publication: Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7. doi: 10.1016/j.molcel.2023.08.005.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 7000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: OPTN
• Alt name: ALS12
• Species: H. sapiens (human)
• Insert Size (bp): 3000
• GenBank ID: NM_001008211
• Entrez Gene: OPTN (a.k.a. ALS12, FIP2, GLC1E, HIP7, HYPL, NRP, TFIIIA-INTP)
• Promoter: CMV
• Tag / Fusion Protein:
  • Halo (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: ApaI (not destroyed)
• 5′ sequencing primer: pdHT 5'-GTCGAAGAATACATGGACTG
• 3′ sequencing primer: NA

Resource Information

• A portion of this plasmid was derived from a plasmid made by: I. Dikic (Goethe University); Wild et al., Science 2011

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2022.06.21.496850v1 for bioRxiv preprint.