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UAS-4x(tRNA::dsarm{sgRNA})
(Plasmid #187885)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187885 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCASPR (attB)
  • Backbone size w/o insert (bp) 9000
  • Total vector size (bp) 10000

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    4 sgRNAs targeting dSarm
  • Alt name
    gRNA: GGGCGGCGGTGGGATGGGTA, GGATAATGGTCTGGATAAGG, GGTTCCCTTGTGGTCCGTGG, GGAGGTGGTGGCGGCGTTAC
  • Insert Size (bp)
    1000

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UAS-4x(tRNA::dsarm{sgRNA}) was a gift from Lukas Neukomm (Addgene plasmid # 187885 ; http://n2t.net/addgene:187885 ; RRID:Addgene_187885)

  • For your References section:

    The NAD(+) precursor NMN activates dSarm to trigger axon degeneration in Drosophila. Llobet Rosell A, Paglione M, Gilley J, Kocia M, Perillo G, Gasparrini M, Cialabrini L, Raffaelli N, Angeletti C, Orsomando G, Wu PH, Coleman MP, Loreto A, Neukomm LJ. Elife. 2022 Dec 7;11:e80245. doi: 10.7554/eLife.80245. 10.7554/eLife.80245 PubMed 36476387
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