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Addgene

pFLAG-Egfr(Velvet)
(Plasmid #18788)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18788 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFLAG-CMV3
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 6280
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    epidermal growth factor receptor
  • Alt name
    Egfr
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3561
  • Mutation
    D833G; signal sequence removed (aa1-24)
  • Entrez Gene
    Egfr (a.k.a. 9030024J15Rik, Erbb, Errb1, Errp, Wa5, wa-2, wa2)
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (not destroyed)
  • 3′ cloning site Bgl II (not destroyed)
  • 5′ sequencing primer N-CMV-30 sequencing primer
  • 3′ sequencing primer C-CMV-24 sequencing primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The EGFR internal signal sequence was removed during the cloning of this construct. When compared to GenBank reference sequence NP_997538.1, the signal sequence corresponds to amino acids 1-24

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFLAG-Egfr(Velvet) was a gift from Bruce Beutler (Addgene plasmid # 18788 ; http://n2t.net/addgene:18788 ; RRID:Addgene_18788)

  • For your References section:

    Velvet, a dominant Egfr mutation that causes wavy hair and defective eyelid development in mice. Du X, Tabeta K, Hoebe K, Liu H, Mann N, Mudd S, Crozat K, Sovath S, Gong X, Beutler B. Genetics. 2004 Jan . 166(1):331-40. 10.1534/genetics.166.1.331 PubMed 15020428
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