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Addgene

pIRE YIC
(Plasmid #18784)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18784 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYIC
  • Backbone manufacturer
    Available at Addgene (#18673)
  • Backbone size w/o insert (bp) 6270
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H-ferritin iron response element
  • Alt name
    IRE
  • Insert Size (bp)
    35

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

An oligonucleotide with one copy of H-ferritin iron response element, IRE, was inserted into the 5'UTR of pYIC DNA to generate a bicistronic reporter gene with IRE: The IRE was inserted at the NheI and SacI sites (pIRE-YIC; 15 nt downstream of transcription start site and 9 nt upstream of YFP translational start site).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRE YIC was a gift from Han Htun (Addgene plasmid # 18784 ; http://n2t.net/addgene:18784 ; RRID:Addgene_18784)

  • For your References section:

    Different modes and potencies of translational repression by sequence-specific RNA-protein interaction at the 5'-UTR. Nie M, Htun H. Nucleic Acids Res. 2006 . 34(19):5528-40. 10.1093/nar/gkl584 PubMed 17023487
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