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pLX304-VHL-IRES-Tdtomato
(Plasmid #187643)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187643 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLX304-gate-IRES-Tdtomato
  • Backbone manufacturer
    Gift from V. Koduri
  • Modifications to backbone
    Modified to express Tdtomato
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VHL
  • Alt name
    Von-Hippel Lindau Disease Tumor Suppressor, E3 Ubiquitin Protein Ligase
  • Species
    H. sapiens (human)
  • Entrez Gene
    VHL (a.k.a. HRCA1, RCA1, VHL1, pVHL)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLX304-VHL-IRES-Tdtomato was a gift from William Kaelin (Addgene plasmid # 187643 ; http://n2t.net/addgene:187643 ; RRID:Addgene_187643)

  • For your References section:

    HIF-independent synthetic lethality between CDK4/6 inhibition and VHL loss across species. Nicholson HE, Tariq Z, Housden BE, Jennings RB, Stransky LA, Perrimon N, Signoretti S, Kaelin WG Jr. Sci Signal. 2019 Oct 1;12(601). pii: 12/601/eaay0482. doi: 10.1126/scisignal.aay0482. 10.1126/scisignal.aay0482 PubMed 31575731
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