pCS2-HA-GAAZFP-FokI-RR
(Plasmid #18754)

• Depositing Lab: Scot Wolfe
• Publication: Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701.

Backbone

• Vector backbone: CS2
• Backbone size w/o insert (bp): 4760
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: zinc finger nuclease
• Species: M. musculus (mouse)
• Insert Size (bp): 267
• Mutation: RNA expression vector for expressing the zinc finger nuclease. Zinc fingers are subcloned between the kpn1 and bamHI sites. This results in a direct, in frame fusion with the C-terminal FokI nuclease. In this case the nuclease is the RR version of the heterodimeric set. In addition there is an N-terminal HA tag.

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: kpnI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CS2 Forward

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry