pWZL hygro 12S E1A
(Plasmid #18748)

• Depositing Lab: Scott Lowe
• Publication: Serrano et al Cell. 1997 Mar 7. 88(5):593-602.

Backbone

• Vector backbone: pWZL Hygro
• Backbone manufacturer: Available at Addgene (#18750)
• Backbone size w/o insert (bp): 5915
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 12S E1A
• Alt name: E1A 12S
• Alt name: E1a protein from 12s mrna
• Species: adenovirus
• Insert Size (bp): 701
• GenBank ID: CAB40664.1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: IRES-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Jay Morgenstern
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry