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VPS13C^halo∆1235-1748
(Plasmid #187297)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187297 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone size w/o insert (bp) 4731
  • Total vector size (bp) 14576
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    32 degree is better to avoid mutagenesis
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    VPS13C truncation mutant
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    10629
  • Entrez Gene
    VPS13C (a.k.a. BLTP5C, PARK23)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    A plasmid containing codon-optimized cDNA encoding full-length human VPS13C, with a Halo protein after amino acid residue 1914 (VPS13C^Halo), was generated by and purchased from GenScript Biotech

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    VPS13C^halo∆1235-1748 was a gift from Pietro De Camilli (Addgene plasmid # 187297 ; http://n2t.net/addgene:187297 ; RRID:Addgene_187297)

  • For your References section:

    In situ architecture of the lipid transport protein VPS13C at ER-lysosome membrane contacts. Cai S, Wu Y, Guillen-Samander A, Hancock-Cerutti W, Liu J, De Camilli P. Proc Natl Acad Sci U S A. 2022 Jul 19;119(29):e2203769119. doi: 10.1073/pnas.2203769119. Epub 2022 Jul 13. 10.1073/pnas.2203769119 PubMed 35858323
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