PLL5.0-Anillin ShRNA-Cherry-AnillinFL
(Plasmid #187271)

• Purpose: Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin full length
• Depositing Lab: Alpha Yap
• Publication: Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12. doi: 10.1016/j.devcel.2019.04.031. Epub 2019 May 16.

Backbone

• Vector backbone: pLL5.0
• Backbone size w/o insert (bp): 7590
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra)
• gRNA/shRNA sequence: TCGGCGATGCCTCTTTGAATAAATTCAAGAGATTTATTCAAAGAGGCATCGCCATTTTTT
• Species: H. sapiens (human)
• Insert Size (bp): 3324
• Entrez Gene: ANLN (a.k.a. FSGS8, Scraps, scra)
• Promoter: U6, CMV
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: mU6 Forward Primer, mCherry-F
• 3′ sequencing primer: WPRE-R

Resource Information

• Supplemental Documents:
  • PLL5.0-Anillin ShRNA-Cherry-AnillinFL.gb
• A portion of this plasmid was derived from a plasmid made by: Plasmid made by Srikanth Budnar

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Rescue with reconstituted FL Anillin.

5' cloning site: SacII (not destroyed), 3' cloning site: Sbf1 (destroyed).