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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 18706 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMCV/myc/mito
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5000
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name8-oxoguanine DNA glycosylase
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SpeciesH. sapiens (human)
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Insert Size (bp)1035
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MutationN/A
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GenBank IDNM_002542
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Entrez GeneOGG1 (a.k.a. HMMH, HOGG1, MUTM, OGH1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer cgcaaatgggcggtag
- 3′ sequencing primer BGH reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV/myc/mito-hOGG1 was a gift from David Sidransky (Addgene plasmid # 18706 ; http://n2t.net/addgene:18706 ; RRID:Addgene_18706) -
For your References section:
Targeting of mutant hogg1 in mammalian mitochondria and nucleus: effect on cellular survival upon oxidative stress. Chatterjee A, Mambo E, Zhang Y, Deweese T, Sidransky D. BMC Cancer. 2006 . 6():235. 10.1186/1471-2407-6-235 PubMed 17018150