pEZY202
(Plasmid #18705)

• Purpose: (Empty Backbone) pEG202 converted Gateway Destination vector for yeast two-hybrid bait vector
• Depositing Lab: Yu-Zhu Zhang
• Publication: Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

Backbone

• Vector backbone: pEG202
• Backbone size (bp): 12414
• Modifications to backbone: Gateway cassette was inserted into original vector to create the pEZY202 Gateway Destination vector
• Vector type: Yeast Expression ; two-hybrid bait vector
• Selectable markers: HIS3

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DB3.1
• Growth instructions: E.coli strain DB3.1, at 30C
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pHybLex Forward (5'-agggctggcggttggggttattcgc-3')
• 3′ sequencing primer: pHybLex Reverse (5'-GAGTCACTTTAAAATTTGTATACAC-3')

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pEG202 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate a bait vector expression clone for yeast two-hybrid.