pEZY45
(Plasmid #18704)

• Purpose: (Empty Backbone) pJG4-5 converted Gateway Destination vector for yeast two-hybrid prey vector
• Depositing Lab: Yu-Zhu Zhang
• Publication: Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

Backbone

• Vector backbone: pJG4-5
• Backbone size (bp): 8718
• Modifications to backbone: Gateway cassette was inserted into original vector to create the pEZY45 Gateway Destination vector
• Vector type: Yeast Expression ; two-hybrid prey vector
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DB3.1
• Growth instructions: E.coli strain DB3.1, at 30C
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None
• Promoter: GAL1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: GAL1 (5'-AATATACCTCTATACTTTAACGTC-3')

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pJG4-5 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate a prey vector expression clone for yeast two-hybrid.