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Addgene

pCF806-shABC1
(Plasmid #186713)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186713 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCF806
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ARAF, BRAF, CRAF
  • gRNA/shRNA sequence
    tttcgtcccttgatgagtcggt, tttgaataaggtaactgtccag, tctctgaaaacatgtgttctgc
  • Species
    H. sapiens (human)
  • Entrez Gene
    BRAF (a.k.a. B-RAF1, B-raf, BRAF-1, BRAF1, NS7, RAFB1)
  • Entrez Gene
    RAF1 (a.k.a. CMD1NN, CRAF, NS5, Raf-1, c-Raf)
  • Entrez Gene
    ARAF (a.k.a. A-RAF, ARAF1, PKS2, RAFA1)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCF806-shABC1 was a gift from Christof Fellmann (Addgene plasmid # 186713 ; http://n2t.net/addgene:186713 ; RRID:Addgene_186713)

  • For your References section:

    Endogenous spacing enables co-processing of microRNAs and efficient combinatorial RNAi. Amen AM, Loughran RM, Huang CH, Lew RJ, Ravi A, Guan Y, Schatoff EM, Dow LE, Emerling BM, Fellmann C. Cell Rep Methods. 2022 Jun 21;2(7):100239. doi: 10.1016/j.crmeth.2022.100239. eCollection 2022 Jul 18. 10.1016/j.crmeth.2022.100239 PubMed 35880017
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