pNL-EGFP/TREPittdU3
(Plasmid
#18659)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 18659 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC18
- Backbone size w/o insert (bp) 8398
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Growth instructionsDH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGFP
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SpeciesAequorea victoria
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Insert Size (bp)761
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI, SacII (unknown if destroyed)
- 3′ cloning site BsrGI (unknown if destroyed)
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDr. Siamak Mohammadi, University of Pittsburgh. E-mail: [email protected]
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNL-EGFP/TREPittdU3 was a gift from Jakob Reiser (Addgene plasmid # 18659 ; http://n2t.net/addgene:18659 ; RRID:Addgene_18659) -
For your References section:
Tight control of transgene expression by lentivirus vectors containing second-generation tetracycline-responsive promoters. Pluta K, Luce MJ, Bao L, Agha-Mohammadi S, Reiser J. J Gene Med. 2005 Jun . 7(6):803-17. 10.1002/jgm.712 PubMed 15655804