pMXs-gw
(Plasmid #18656)

• Depositing Lab: Shinya Yamanaka
• Publication: Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Backbone

• Vector backbone: pMXs
• Backbone size w/o insert (bp): 4750
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): ccdB Survival
• Growth instructions: DB3.1
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Gateway cassette A
• Alt name: attR
• Insert Size (bp): 1750

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: EcoRI (destroyed during cloning)
• 3′ cloning site: XhoI (destroyed during cloning)
• 5′ sequencing primer: pMX-S1811

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H. Gateway cassette is from Invitrogen Corporation.
• Articles Citing this Plasmid:
  • 14 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The sequence of primer pMX-S1811 is GAC GGC ATC GCA GCT TGG ATA CAC.