pCambia-PUP-IT
(Plasmid
#186478)
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PurposeExpresses POI-PUP-IT fusion in Plants
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 186478 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePcambia 1300
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Vector typePlant Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFLAG-Pup(E)
- Promoter 35S
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Tag
/ Fusion Protein
- Triple Flag Tag
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CAGGAAACAGCTATGAC
- 3′ sequencing primer ACTGGCCGTCGTTTTAC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCambia-PUP-IT was a gift from Jen Sheen (Addgene plasmid # 186478 ; http://n2t.net/addgene:186478 ; RRID:Addgene_186478) -
For your References section:
Glucose-driven TOR-FIE-PRC2 signalling controls plant development. Ye R, Wang M, Du H, Chhajed S, Koh J, Liu KH, Shin J, Wu Y, Shi L, Xu L, Chen S, Zhang Y, Sheen J. Nature. 2022 Sep 14. pii: 10.1038/s41586-022-05171-5. doi: 10.1038/s41586-022-05171-5. 10.1038/s41586-022-05171-5 PubMed 36104568
Map uploaded by the depositor.
