2Bc-T MBP_BoMoC(ed)_6xH
(Plasmid #186461)

• Purpose: Recombinant B. mori truncated R2 RT expression
• Depositing Lab: Kathleen Collins
• Publication: Upton et al Proc Natl Acad Sci U S A. 2021 Oct 19;118(42). pii: 2107900118. doi: 10.1073/pnas.2107900118.

Backbone

• Vector backbone: pET His6 LIC cloning vector (2Bc-T)
• Backbone manufacturer: Scott Gradia
• Backbone size w/o insert (bp): 5899
• Total vector size (bp): 8419
• Modifications to backbone: Modified to include an N-terminal MBP tag
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: B. mori truncated R2 RT
• Alt name: BoMoC
• Species: Bombyx mori
• Insert Size (bp): 2520
• Promoter: T7
• Tags / Fusion Proteins:
  • MBP (N terminal on insert)
  • 6xHis (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry