pBAD-iGECInano
(Plasmid
#186189)
-
PurposeExpression of small FRET-based near-infrared genetically-encoded calcium indicator in bacteria
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 186189 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepBAD/HisB vector
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameiGECInano
-
SpeciesSynthetic
- Promoter AraC
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pBAD-iGECInano was a gift from Vladislav Verkhusha (Addgene plasmid # 186189 ; http://n2t.net/addgene:186189 ; RRID:Addgene_186189) -
For your References section:
Design and Initial Characterization of a Small Near-Infrared Fluorescent Calcium Indicator. Matlashov ME, Vera J, Kasatkina LA, Khodakhah K, Verkhusha VV. Front Cell Dev Biol. 2022 Jun 29;10:880107. doi: 10.3389/fcell.2022.880107. eCollection 2022. 10.3389/fcell.2022.880107 PubMed 35846350