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Addgene

PE-P3-rice
(Plasmid #185612)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185612 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCambia
  • Total vector size (bp) 18409
  • Vector type
    CRISPR
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3XFLAG-4XNLS-M-MLV-nCas9(H840A)-4XNLS-P2A-Hyg
  • Insert Size (bp)
    7671
  • Promoter ZmUbi

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gatcttgatatacttggatgatggc
  • 3′ sequencing primer tctagtaacatagatgacaccgcgc
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PE-P3-rice was a gift from Jinxiao Yang (Addgene plasmid # 185612 ; http://n2t.net/addgene:185612 ; RRID:Addgene_185612)

  • For your References section:

    A design optimized prime editor with expanded scope and capability in plants. Xu W, Yang Y, Yang B, Krueger CJ, Xiao Q, Zhao S, Zhang L, Kang G, Wang F, Yi H, Ren W, Li L, He X, Zhang C, Zhang B, Zhao J, Yang J. Nat Plants. 2022 Jan;8(1):45-52. doi: 10.1038/s41477-021-01043-4. Epub 2021 Dec 23. 10.1038/s41477-021-01043-4 PubMed 34949802
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