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Addgene

pAAV-CAG-EBFP2-P2A-EGFP
(Plasmid #184938)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184938 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-CAG-EGFP-WPRE
  • Backbone size w/o insert (bp) 4723
  • Total vector size (bp) 6250
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EBFP2-P2A-EGFP
  • Species
    Synthetic
  • Insert Size (bp)
    1527
  • Promoter CAG

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CAAAGAATTGGATCCGGTACCGCCACCatggtgagcaagggcgaggag
  • 3′ sequencing primer CCACTTCCCACGTGAACCGGTCGcttgtacagctcgtccatgcc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CAG-EBFP2-P2A-EGFP was a gift from Kiryl Piatkevich (Addgene plasmid # 184938 ; http://n2t.net/addgene:184938 ; RRID:Addgene_184938)

  • For your References section:

    Dual-expression system for blue fluorescent protein optimization. Papadaki S, Wang X, Wang Y, Zhang H, Jia S, Liu S, Yang M, Zhang D, Jia JM, Koster RW, Namikawa K, Piatkevich KD. Sci Rep. 2022 Jun 17;12(1):10190. doi: 10.1038/s41598-022-13214-0. 10.1038/s41598-022-13214-0 PubMed 35715437
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