pTubuiln-Electra1
(Plasmid #184929)

• Purpose: Tubulin labeling with blue fluorescent protein Electra1
• Depositing Lab: Kiryl Piatkevich
• Publication: Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 10.1038/s41598-022-13214-0.

Backbone

• Vector backbone: pcDNA
• Backbone size w/o insert (bp): 5381
• Total vector size (bp): 7508
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TUBA1B-Electra1
• Species: Synthetic
• Insert Size (bp): 2127
• GenBank ID: NM_006082.3 OL631606
• Promoter: CMV
• Tag / Fusion Protein:
  • Electra1 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: bGHp(A)

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry