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PurposeStable expression of HaloTag7-mGFP-KDEL in mammalian cells to measure ER-phagy activity
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 184904 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMRX-IB
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Vector typeMammalian Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameKDEL
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Insert Size (bp)12
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Tags
/ Fusion Proteins
- HaloTag7 (N terminal on insert)
- monomeric EGFP (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMRX-IB-HaloTag7-mGFP-KDEL was a gift from Noboru Mizushima (Addgene plasmid # 184904 ; http://n2t.net/addgene:184904 ; RRID:Addgene_184904) -
For your References section:
A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag. Yim WW, Yamamoto H, Mizushima N. Elife. 2022 Aug 8;11. pii: 78923. doi: 10.7554/eLife.78923. 10.7554/eLife.78923 PubMed 35938926
