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pMRX-No-HaloTag7-mGFP-LC3-mRFP
(Plasmid #184902)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184902 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMRX-No
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LC3B
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    357
  • Tags / Fusion Proteins
    • HaloTag7 (N terminal on insert)
    • monomeric EGFP (N terminal on insert)
    • monomeric RFP (C terminal on insert)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMRX-No-HaloTag7-mGFP-LC3-mRFP was a gift from Noboru Mizushima (Addgene plasmid # 184902 ; http://n2t.net/addgene:184902 ; RRID:Addgene_184902)

  • For your References section:

    A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag. Yim WW, Yamamoto H, Mizushima N. Elife. 2022 Aug 8;11. pii: 78923. doi: 10.7554/eLife.78923. 10.7554/eLife.78923 PubMed 35938926
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