pScalps Luciferase Zsgreen
(Plasmid
#184784)
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PurposeLentiviral vector with luciferase gene under SFFV promoter and Zsgreen under Cyclophilin promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 184784 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePscalps Zsgreen
- Backbone size w/o insert (bp) 7833
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Vector typeLentiviral, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFirefly Luciferase and Zsgreen
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SpeciesFirefly
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Insert Size (bp)1653
- Promoter SFFV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pScalps Luciferase Zsgreen was a gift from Ajit Chande (Addgene plasmid # 184784 ; http://n2t.net/addgene:184784 ; RRID:Addgene_184784) -
For your References section:
SARS CoV-2 Nucleoprotein Enhances the Infectivity of Lentiviral Spike Particles. Mishra T, Sreepadmanabh M, Ramdas P, Sahu AK, Kumar A, Chande A. Front Cell Infect Microbiol. 2021 Apr 23;11:663688. doi: 10.3389/fcimb.2021.663688. eCollection 2021. 10.3389/fcimb.2021.663688 PubMed 33968806
