Activin A (S2-2)
(Plasmid
#184621)
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Purposefor transfection into S2 cells and expression of Activin A protein
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 184621 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepExpreS2-2
- Backbone size w/o insert (bp) 2799
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Vector typeInsect Expression
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Selectable markersG418
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameINHBA
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Alt nameActivin A
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SpeciesH. sapiens (human)
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Insert Size (bp)1496
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Entrez GeneINHBA (a.k.a. EDF, FRP)
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Tag
/ Fusion Protein
- 8x His - streptavidin binding peptide (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Xba1 (unknown if destroyed)
- 3′ cloning site Not1 (unknown if destroyed)
- 5′ sequencing primer CGT CTA CGG AGC GAC AAT TC
- 3′ sequencing primer CAC GCG CTT GAA AGG AGT GT (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Activin A (S2-2) was a gift from Timothy Springer (Addgene plasmid # 184621 ; http://n2t.net/addgene:184621 ; RRID:Addgene_184621) -
For your References section:
Protection of the Prodomain alpha1-Helix Correlates with Latency in the Transforming Growth Factor-beta Family. Le VQ, Iacob RE, Zhao B, Su Y, Tian Y, Toohey C, Engen JR, Springer TA. J Mol Biol. 2022 Mar 15;434(5):167439. doi: 10.1016/j.jmb.2021.167439. Epub 2022 Jan 4. 10.1016/j.jmb.2021.167439 PubMed 34990654