pCMV-3XFLAG-renilla-luciferase-beta-globin-(39PTC)
(Plasmid #184394)

• Purpose: Transient transfection plasmid expressing Renilla-luciferase-beta-globin fusion protein with PTC in beta-globin
• Depositing Lab: Robert Bradley
• Publication: Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii: 5/3/e202101217. doi: 10.26508/lsa.202101217. Print 2022 Mar.

Backbone

• Vector backbone: p3xFLAG-CMV-10
• Backbone size w/o insert (bp): 7627
• Total vector size (bp): 8557
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Renilla luciferase
• Species: Renilla reniformis
• Insert Size (bp): 930
• Promoter: CMV
• Tag / Fusion Protein:
  • 3X FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: TGACGCAAATGGGCGGTAG

Resource Information

• Supplemental Documents:
  • pCMV-3XFLAG-renilla-luciferase-beta-globin-(39PTC).gbk
• A portion of this plasmid was derived from a plasmid made by: The Renilla luciferase sequence was synthesized by Genscript and amplified via PCR to be cloned into the backbone.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The backbone is from a plasmid from James Inglese's lab (Addgene catalog # 112084, published in PMID: 29528287). The Renilla luciferase sequence replaces the firefly luciferase sequence in that plasmid. Additional cloning details are available in Udy et al., 2021 (PMID: 34880103).