pRP[2CRISPR]-mCherry/Hygro-hCas9-U6>{mdx left}-U6>{mdx right}
(Plasmid #184381)

• Purpose: This plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23
• Depositing Lab: Ori Bar-Nur
• Publication: Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-336. doi: 10.1016/j.stemcr.2021.12.003. Epub 2022 Jan 6.

Backbone

• Vector backbone: n/a
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cas9, two gRNAs targeting dystrophin
• gRNA/shRNA sequence: TCTTTGAAAGAGCAATAAAA, ATTTCAGGTAAGCCGAGGTT
• Species: M. musculus (mouse)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • pRP[2CRISPR]-mCherryHygro-hCas9-U6{mdx left}-U6{mdx right}.gbk
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid was generated by VectorBuilder, ID: VB190715-1035jad