FZ-NbPDS
(Plasmid #184268)

• Purpose: pAIDE containing a full-length CPSMV RNA2 cDNA modified to accomodate an NbPDS fragment.
• Depositing Lab: Feng Qu
• Publication: Zaulda et al Plant Methods. 2022 Oct 28;18(1):116. doi: 10.1186/s13007-022-00950-7.

Backbone

• Vector backbone: pAIDE (a pCambia1300 drivative)
• Backbone manufacturer: Qu lab
• Backbone size w/o insert (bp): 7872
• Total vector size (bp): 12030
• Vector type: Plant Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CPSMV RNA2 cDNA, modified to permit foreign gene insertion between MP and L-CP
• Species: Cowpea severe mosaic virus
• Insert Size (bp): 4158
• Mutation: The protease processing site between MP and L-CP duplicated.
• GenBank ID: NC_003544.1
• Promoter: CaMV 35S with duplicated enhancer

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GAAACGACAATCTGATCCAAGCTCAA
• 3′ sequencing primer: GAGTTAGCTCACTCATTAGGCAC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.researchsquare.com/article/rs-1463088/v1 for preprint.