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Addgene

Anti-TrpC5 [N67/15R]
(Antibody #184205)

Ordering

Item Catalog # Description Quantity Price (USD)
Recombinant Antibody 184205-rAb 100 µg of purified recombinant antibody $250 *
Recombinant Antibody trial size 184205-rAb.T 20 µg of purified recombinant antibody $85 *

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Features

Production & Usage

  • Production Plasmid
    Anti-TrpC5 [N67/15R]
  • Purification
    Purified from cell culture supernatant using Protein A or Protein G affinity columns followed by a buffer exchange with centrifugal columns.
  • Storage Buffer
    PBS + 1 mM sodium azide

Delivery

  • Concentration
    0.9–1.1 mg/mL
  • Storage
    Upon receipt, prepare single use aliquots and store at -20 °C until use. Avoid multiple freeze thaw cycles.
  • Shipment
    Blue ice

Resource Information

  • Persistent Resource Identifier
    RRID:AB_2895711

Terms and Licenses

Target Antigen

Protein Short transient receptor potential channel 5
Antigen Description Amino acids 827–845 of human TrpC5
Antigen Amino Acid Sequence
SKAESSSKRSFMGPSLKKL
Species H. sapiens (human)
Additional Species Reactivity H. sapiens (human),  M. musculus (mouse)
Gene TRPC5
Alternative Names
  • Transient receptor protein 5
  • TRP-5
  • hTRP-5
  • hTRP5
External References

Applications (2)

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Immunohistochemistry

1 image
Immunohistochemistry image by Zhuhao Wu, Weill Cornell Medicine
Submitted By: Zhuhao Wu, Weill Cornell Medicine
Addgene Partner
Antibody Type
Recombinant
Sample Species
M. musculus (mouse)
Result
Pass

Western Blot

1 image
Western Blot image by David Clapham, HHMI/Harvard Medical School Knockout validation
Submitted By: David Clapham, HHMI/Harvard Medical School
Antibody Type
Hybridoma
Sample Species
M. musculus (mouse)
Result
Pass
How to cite this antibody ( Back to top)

These antibodies were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the antibodies were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Anti-TrpC5 [N67/15R] - from James Trimmer (Addgene antibody # 184205 ; http://n2t.net/addgene:184205 ; RRID:AB_2895711)
  • For your References section:

    A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. Andrews NP, Boeckman JX, Manning CF, Nguyen JT, Bechtold H, Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS. Elife. 2019 Jan 22;8. pii: 43322. doi: 10.7554/eLife.43322. 10.7554/eLife.43322 PubMed 30667360