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Addgene

GST-SmB'
(Plasmid #1842)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1842 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-2TK
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 4969
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SmB'
  • Alt name
    splicing factor
  • Alt name
    snRNP protein
  • Alt name
    small nuclear ribonucleoprotein polypeptides B and B1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    100
  • Entrez Gene
    SNRPB (a.k.a. CCMS, COD, SNRPB1, Sm-B/B', SmB/B', SmB/SmB', snRNP-B)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pGEX 5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

(Leder #C-1092)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST-SmB' was a gift from Philip Leder (Addgene plasmid # 1842 ; http://n2t.net/addgene:1842 ; RRID:Addgene_1842)

  • For your References section:

    WW domain-mediated interactions reveal a spliceosome-associated protein that binds a third class of proline-rich motif: the proline glycine and methionine-rich motif. Bedford MT, Reed R, Leder P. Proc Natl Acad Sci U S A 1998 Sep 1;95(18):10602-7. 10.1073/pnas.95.18.10602 PubMed 9724750
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