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Addgene

sgDonor_lentiGuide
(Plasmid #184168)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184168 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    lentiGuide-Puro
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgDonor
  • gRNA/shRNA sequence
    AAGAGCGAATCGATTTCGTG

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    sgDonor_lentiGuide was a gift from Ophir Shalem (Addgene plasmid # 184168 ; http://n2t.net/addgene:184168 ; RRID:Addgene_184168)

  • For your References section:

    Efficient and flexible tagging of endogenous genes by homology-independent intron targeting. Serebrenik YV, Sansbury SE, Kumar SS, Henao-Mejia J, Shalem O. Genome Res. 2019 Aug;29(8):1322-1328. doi: 10.1101/gr.246413.118. Epub 2019 Jun 25. 10.1101/gr.246413.118 PubMed 31239279
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