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Addgene

mCherry-BRD4
(Plasmid #183939)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183939 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEG(/Y)FP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4203
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry-BRD4
  • Alt name
    bromodomain containing
  • Alt name
    mCherry
  • Species
    M. musculus (mouse), Synthetic
  • Mutation
    none
  • Entrez Gene
    Brd4 (a.k.a. Brd5, HUNK1, MCAP, WI-11513)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site BsrGI (not destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry-BRD4 was a gift from Karsten Rippe (Addgene plasmid # 183939 ; http://n2t.net/addgene:183939 ; RRID:Addgene_183939)

  • For your References section:

    Transcription activation is enhanced by multivalent interactions independent of phase separation. Trojanowski J, Frank L, Rademacher A, Mucke N, Grigaitis P, Rippe K. Mol Cell. 2022 May 4. pii: S1097-2765(22)00328-8. doi: 10.1016/j.molcel.2022.04.017. 10.1016/j.molcel.2022.04.017 PubMed 35537448
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