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Addgene

NLS-HA-tdMCP-tdTomato
(Plasmid #183938)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183938 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    tdTomato-N1
  • Backbone manufacturer
    Addgene #54642
  • Backbone size w/o insert (bp) 5443
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tdMCP-tdTomato
  • Alt name
    tandem MS2 coat protein
  • Species
    Synthetic
  • Insert Size (bp)
    810
  • Mutation
    none
  • Entrez Gene
    cp (a.k.a. MS2g2)
  • Promoter CMV (TATA box removed)
  • Tag / Fusion Protein
    • NLS-HA (N terminal on insert)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

under control of the weakened deltaTATA-CMV promoter for low level expression

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NLS-HA-tdMCP-tdTomato was a gift from Karsten Rippe (Addgene plasmid # 183938 ; http://n2t.net/addgene:183938 ; RRID:Addgene_183938)

  • For your References section:

    Transcription activation is enhanced by multivalent interactions independent of phase separation. Trojanowski J, Frank L, Rademacher A, Mucke N, Grigaitis P, Rippe K. Mol Cell. 2022 May 4. pii: S1097-2765(22)00328-8. doi: 10.1016/j.molcel.2022.04.017. 10.1016/j.molcel.2022.04.017 PubMed 35537448
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