NLS-tdPCP-CIBN
(Plasmid #183937)

• Purpose: PCP tandem dimer binding to PP7 RNA stem loops coupled to optogenetic CIBN domain; bound by PHR upon blue light exposure
• Depositing Lab: Karsten Rippe
• Publication: Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22)00328-8. doi: 10.1016/j.molcel.2022.04.017.

Backbone

• Vector backbone: tdTomato-N1
• Backbone manufacturer: Addgene #54642
• Backbone size w/o insert (bp): 4592
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: tdPCP-CIBN
• Alt name: tandem PP7 coat protein
• Alt name: CIB1, N-terminal domain
• Species: A. thaliana (mustard weed), Synthetic
• Mutation: none
• Entrez Gene: CIB1 (a.k.a. AT4G34530, T4L20.110, T4L20_110, cryptochrome-interacting basic-helix-loop-helix 1)
• Promoter: CMV (TATA box removed)
• Tag / Fusion Protein:
  • NLS (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: AgeI (not destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

under control of the weakened deltaTATA-CMV promoter for low level expression